Fig. 6. Assembly of chimeric TRPV channel subunits. (A) Chimeric TRPV channel subunits were constructed consisting of various combinations of the cytosolic N- or C-termini and the transmembrane domains of TRPV1 and TRPV4. (B-D) HEK293 cells were transiently co-transfected with expression plasmids encoding CFP- or YFP-fused TRPV channels or TRPV channel chimeras as indicated. FRET efficiencies (means±s.e.) were determined as described in Fig. 3. Grey bars, FRET efficiencies of only full-length TRPV channel subunits; black bars, FRET experiments with TRPV channel chimeras. [YFP]_r and [CFP]_r represent means of the CFP and YFP fluorescence intensities.