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Fig. 1. Distribution of Hic-5 in adult mouse tissues. (A) Immunoreactivity with anti-Hic-5 monoclonal antibody (middle), anti-{alpha}-SM-actin monoclonal antibody (bottom) and control IgG (top) in the aorta (left) and the large intestine (right). (B) The colocalization of endogenous and transfected Hic-5. The cells were transfected with the expression vector of HA-tagged Hic-5 (pCG-LD1mhic-5) subjected to uni-axial cyclic stretch at 1 Hz for 60 minutes (stretch +) or not (stretch –) and immunostained with both anti-HA (Y-11) antibody and anti-Hic-5 antibody. The anti-Hic-5 antibody stained the same types of structures in the transfected as in the neighboring untransfected cells, supporting the colocalization of the endogenous and exogenous proteins. The arrow indicates the direction of stretch. Bars, 5 µm.