Fig. 4. Intracellular distribution of CRP2 and -actinin during uni-axial cyclic stretch. Mouse embryo fibroblast cells were transfected with Flag-tagged CRP2 expression vector, subjected to cyclic stretching (+) or not (–) and stained with the polyclonal antibody against the Flag tag (A) or with that against endogenous -actinin (B). The insets in the merged images are magnification of a part of a cell's rectangular frame. The white arrows in the inset of B show a periodic pattern of -actinin staining. Bars, 10 µm. (C) The Hic-5 domains interacting with the factors and presence (+) or absence (–) of the factors on stress fiber during the cyclic stretching.