Fig. 5. The C-terminal region of Hic-5 is sufficient for the stress-fiber-restricted localization under cyclic stretching. (A) The HA-tagged Hic-5 deletion mutants used in this experiment. (B) The expression plasmids of the Hic-5 mutants were transfected into mouse embryo fibroblast cells; 24 hours later, western blotting was carried out using the antibody to the HA tag. (C) Mouse embryo fibroblast cells were transfected with each expression vector, subjected to cyclic stretching (+) or not (–) and immunostained with the 12CA5 antibody against the HA tag (left, red). (C, middle) F-actin stained by FITC-conjugated phalloidin (green); (C, right) merged images. The arrow indicates the direction of stretching. Bars, 5 µm.