Fig. 2. Heterochromatic association in CNS nuclei from developmentally arrested larvae. (A) The effect of feeding larvae erg2 yeast. Larvae were reared on wild-type yeast (top) or erg2 yeast (bottom). Irrespective of the yeast fed, the larvae enter the mid-third instar larval stage and appear to undergo normal development until 115 hours AED. At 135 hours AED the larvae fed erg2 yeast remain stalled in the third instar stage and eventually die after a few more days. By this time, larvae fed wild-type yeast have pupated (they usually pupate at 118-125 hours AED) and are ready to eclose 216 hours AED. (B) Box plots (as in Fig. 1) show data from FISH on CNS from bw⁺ and bw^D larvae. Larvae fed erg2 yeast were dissected at 110-115 hours AED (day 1) or 130-135 hours AED (day 2). Larvae fed wild-type (WT) yeast were dissected at 110-115 hours AED (day 1). P values (Mann-Whitney) are shown above brackets for the respective sets. (C) Histograms showing the distribution of the distances between 59E and 2Rh in bw^D larvae reared on WT yeast on day 1 and erg2 yeast on day 2.