Fig. 1. (A) PEDF dose-dependently inhibits FGF-2-induced capillary morphogenesis of IBE cells. IBE cells were suspended in Ham's F-12 medium containing 0.25% BSA and seeded onto growth-factor-reduced Matrigel. Cells were cultured with or without 20 ng ml^–1 FGF-2 in the presence or absence of PEDF at indicated concentrations for 24 hours. Photographs were taken under a phase-contrast microscope. Relative tube length was expressed as means±s.d. for three photographs. Bar, 100 µm. (B) PEDF inhibits FGF-2-induced capillary morphogenesis of HUVECs. HUVECs were suspended in EBM-2 containing 0.5% FBS and seeded onto Matrigel. Indicated samples were added to the cells and culture was continued for 24 hours. Photographs were taken under a phase-contrast microscope. Relative tube length was expressed as means±s.d. for three photographs. Bar, 100 µm. Reproducible results were obtained from two or three independent experiments.