Fig. 5. Expression of constitutively active Fyn in IBE cells restores the PEDF-mediated inhibition of capillary morphogenesis. (A) Two stable clones, CAFyn-6 and CAFyn-12, ectopically express Myc-tagged constitutively active Fyn. Empty-vector-transfected cells (Mock cells) were used as a negative control. (B) CAFyn-6 and CAFyn-12 cells cause FGF-2-independent capillary morphogenesis and its morphology is resistant to PEDF treatment. Cells were seeded onto Matrigel and cultured for 24 hours in the presence or absence of the indicated samples. Photographs were taken under a phase-contrast microscope. Relative tube length was expressed as means±s.d. for three photographs. Bar, 100 µm. Reproducible results were obtained from two or three independent experiments.