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Fig. 2. CPEB1 localization in HeLa cells. (A) Western blot assay. The anti-CPEB1 monoclonal antibody 2B7 was used for western blotting of proteins from HeLa cells expressing the murine CPEB1-{Delta}5-short or the human CPEB1-{Delta}5-long isoform. Untransfected HeLa cells were used as a control. (B) Immunofluorescence of untransfected HeLa cells. Cells were fixed, stained with anti-CPEB1 antibody and observed by fluorescence microscopy. Bar, 8 µm.