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Fig. 5. Small CPEB1 foci depend on translation. (A) HeLa/CPEB1-lg cells were induced for 16 hours with doxycycline, treated with cycloheximide (CHX) for 40 minutes or puromycin for 1 hour, then fixed and observed by fluorescence microscopy. (B) The number of CPEB1 foci per cell was counted in 150 control, cycloheximide- and puromycin-treated cells.