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Fig. 6. Polyclonal antibody BUGS and pAb SuperBUGS recognise native MAP1B in E12 rat embryos. Western immunoblots of tissue from an E12 rat embryo probed with antibodies against MAP1B (mAb AA6) and GSK-3ß phosphorylated MAP1B (mAb SMI-31, pAb BUGS and pAb SuperBUGS). Monoclonal antibody AA6 recognises a single, high molecular weight protein band corresponding to MAP1B. Monoclonal antibody SMI-31 also recognises MAP1B but, in addition, several lower molecular weight protein bands probably corresponding to neurofilament and nuclear epitopes (Lichtenberg-Kraag et al., 1992). Polyclonal antibody BUGS and pAb SuperBUGS recognise a single protein band that co-migrates with MAP1B (arrowhead). Molecular weights (kDa) are indicated on the left hand side.