Fig. 5. Cathodal Golgi polarization and electrotaxis of CHO cells in an EF. (A) Time course of directional cell migration, morphologic polarization and Golgi redistribution. (B) Correlation of Golgi polarization with morphologic polarization and directedness of cell migration. (C) Correlation of Golgi polarization with migration rates in an EF. In B and C, CHO cells were pretreated as indicated with BFA (1 µM, 5 µM), CyD (0.2 µM), PP2 (20 µM), Y27632 (50 µM), Toxin B (10 ng/ml) or LS203 (100 ng/ml) for 2 hours and subjected to an EF for 3 hours with presence of the drug. Cell migration, morphologic polarization and Golgi polarization were analysed as in Materials and methods. Each data point in A represents mean±s.e.m. from at least 200 cells from three independent experiments.