Fig. 8. An electric field of 300 mV/mm predominates over other directional cues in directing cell migration in the scratch wound model. Migration trajectories of individual cells are indicated by red lines and direction and endpoint of a 3-hour experiment indicated by arrows. (A) No EF control, wound edge moved into the wound. (B) an EF applied with the field vector in normal healing direction enhanced cell migration into the wound (P<0.001). (C) An EF applied with the field vector opposite to the normal healing direction directed the cells at the wound edge to migrate away from the wound. BFA significantly decreased but did not completely abolish directional cell migration induced by wounding and/or an applied EF (D-G). Directness was calculated from 60 cells collected from three independent experiments (G). See also supplementary material video 3.