Fig. 2. PITX2 isoforms differentially regulate the LEF-1 promoter. CHO cells were transfected as described in Fig. 1, using the three major PITX2 isoforms. The activities are shown as mean fold activation compared with the LEF-1 promoter plasmid without PITX2 expression and normalized to ß-galactosidase activity (± s.e.m. from four independent experiments).