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Fig. 5. LEF-1 binds to the C-terminal tail of PITX2. (A) The PITX2 deletion constructs used to map the LEF-1 interaction. (B) GST-PITX2A, GST-PITX2 HD (homeodomain only), GST-PITX2 C173 (C-terminal tail only) and GST-PITX2 {Delta}173 (deletion of the C-terminal tail) pull-down assay with bacterial expressed and purified LEF-1 protein (50 ng). LEF-1 binds to GST-PITX2A and GST-PITX2 C173 but not to GST-PITX2 HD or GST-PITX2 {Delta}173. The bound protein was detected by western blot using a LEF-1 antibody. As a control GST-beads were incubated with purified LEF-1 to show the specificity of binding to PITX2A.