Fig. 2. Sec5 protein is mislocalized in sec6 excision alleles. (A) Ventral nerve cords from yw larvae at 24 hours and 72 hours AEL, and sec6^Ex15/+, sec6^Ex212/+, sec6^Ex15, sec6^Ex212 and sec6^Ex15/Ex212 at 72 hours AEL. All nerve cords were stained for Sec5 with monoclonal antibody 22A2. All animals were imaged at the same image settings for comparison. (B) Close ups of the cell body and neuropil regions of the nerve cords from A. (C) Quantitation of mean pixel intensity for a fixed area of the neuropil region of nerve cords from yw 24 hours (n=6), yw 72 hours (n=4), sec6^Ex15/+ 72 hours (n=4), sec6^Ex212/+ 72 hours (n=5), sec6^Ex15 72 hours (n=8), sec6^Ex212 72 hours (n=5) and sec6^Ex15/Ex212 72 hours (n=8). Statistical significance of the reduction of Sec5 in the mutant neuropil compared with the wild type is: P=0.008 (sec6^Ex15 72 hours vs yw 24 hours); P=0.04 (sec6^Ex212 72 hours vs yw 24 hours); P=0.07 (sec6^Ex15/Ex212 72 hours vs yw 24 hours). (D) Ventral nerve cords from yw 24 hours AEL and sec6^Ex15/sec6^Ex212 72 hours AEL were stained for cysteine string protein (CSP) and FasII. All scale bars are 20 µm.