Fig. 5. The sec5^E10 and sec6^Ex15 mutants have similar phenotypes in the ovary. (A-G) Egg chambers labeled with phalloidin (red), Hoechst 33342 (blue) and GFP (green in G). Unlike in the wild type (A), egg chambers from sec5^E10 germ-line clones (B,C) lack membranes (marked with phalloidin) between nuclei and have ring canals clumped together (arrows). Egg chambers from sec6^Ex15 germ-line clones (D-G) exit the germarium and progress through stage 3 (D,E), initially resembling the control. However, after stage 3 (F,G), ring canals clump together (arrows) and phalloidin-marked membranes between nuclei are absent. FRT42D sec6^Ex15 homozygous germ lines were generated by mitotic recombination in combination with either FRT42B ovo^D (D-F) or FRT42D Ubi-GFP and imaging egg chambers that lacked GFP in the germ line (G). (H-L) Egg chambers labeled with Texas Red-phalloidin (red), Hoechst 33342 (blue) and anti-Gurken antibody (green). Gurken accumulates only in the oocyte, which resides at the posterior end of the egg chamber throughout oogenesis, contacting the posterior follicle cells in the wild type (H). In egg chambers from sec5^E10 (I,J) and sec6^Ex15 (K,L) germ-line clones, the oocyte is often mispositioned anteriorly (arrows). Anterior (A) and posterior (P) ends of the chamber are marked. All scale bars are 20 µm.