Fig. 2. Effects of S1P on stress fibre and focal adhesion formation. Untreated C2C12 myoblasts (A) and treated with 1 µM S1P (B) were fixed and double stained with TRITC-phalloidin (red) and anti-vinculin (green). S1P induces formation of massive stress fibres coincident with the redistribution of vinculin-rich focal adhesion sites at the end of stress fibres. The images are representative of at least three independent experiments with similar results.