Fig. 8. Effects of S1P and of the inhibition of Rho kinase, PLD and Gi-coupled receptor on membrane current and conductance. (A-E) Voltage-clamp step pulses as in Fig. 7. Representative current traces (A-E) and normalized I_m versus V_m plots (F) after S1P stimulation in: empty vector transfected myoblast (A, in F); DN-PLD-transfected myoblasts (B, in F); Y-27362-treated myoblasts (C, in F); DN-PLD-transfected myoblasts in the presence of Y-27362 (D, in F); PTx-treated myoblasts (A, in F). The best linear regression lines are superimposed on the data (continuous lines). For comparison, the data and linear regression (dashed line) of control from Fig. 7 are reported. The calculated C_m, G_m and G_m/C_m values for all experimental conditions are reported in Table 2. Data are the mean±s.e.m.