Fig. 10. MMP-1 overexpression following Cdc42 silencing is observed in various human cell lines. Representative western-blot analysis of cell lysates of human breast adenocarcinoma cell line HS578T, fibrosarcoma cell line HT1080 and melanoma cell line A2058 72 hours after transfection, and of serum-free medium conditioned by the same cells for 16 hours between days 2 and 3 after transfection (C.M.). Cells were transfected with 20 nM irrelevant siRNA (siScr) or 20 nM first siRNA targeting Cdc42 (siCdc42). Cell lysates and C.M. were analysed by immunoblotting with specific antibodies to Cdc42, ERK1/2 and MMP-1.