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Fig. 2. Morphological effects of RhoA, Rac1 or Cdc42 silencing in HSFs. Representative phase-contrast microscopy of HSFs 72 hours after transfection with calcium phosphate alone (CaP), 20 nM irrelevant siRNA (siScr) or with 20 nM siRNA targeting RhoA (siRhoA), Rac1 (siRac1) or Cdc42 (siCdc42). Bar, 20 µm.