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Fig. 10. Two pools of Bet3 in the cytosol as revealed by gel filtration analysis. (A) Rat liver cytosol (panels a and b) or denatured cytosol (panels c and d) was resolved by gel filtration and the resulting fractions were analyzed by immunoblotting to detect Bet3 (panels a and c) or ß-COP (panels b and d). (B) GST-Bet3 alone (panel a) or GST-Bet3 pre-incubated with Bet3-depleted rlc (panel b) were resolved by gel filtration and the resulting fractions were analyzed by immunoblotting to detect GST-Bet3. Also shown is the distribution of Bet3 in control cytosol (panel c).