Fig. 9. Bet3 is functionally required after Sec13 but before Rab1 and -SNAP. Semi-intact NRK cells were incubated in transport cocktail with the indicated immunodepleted cytosols for 90 minutes at 32°C (stage I). The cells were subsequently washed by centrifugation and resuspended in fresh transport cocktail supplemented with the indicated immunodepleted cytosols for an additional 45 minutes (stage II). The extent of VSV-G transported to the Golgi was quantified (B).