Fig. 2. Expression levels of 5, v and ß1 integrins in FGFR2 mutant and control (wild type) osteoblasts. (A) Cells were subjected to western blot analysis using specific antibodies against 5, v and ß1 integrins. Equal loading was confirmed by detecting levels of ß-actin. (B) Expression of transcripts for 5, v and ß1 integrins was determined by RT-PCR analysis and GAPDH was used as an internal control. (C) Immunohistochemical analysis of 5 integrin subunit in a normal fetal human coronal suture in vivo. 5 integrin subunit immunoreactivity was localized in osteoblasts (arrows) along the bone matrix. Control sections incubated with IgG showed no specific staining (original magnification, x125).