Fig. 3. The 5 integrin subunit interacts with FGFR2 in osteoblasts. Cell lysates from control and FGFR2 mutant osteoblast cells were immunoprecipitated (IP) with 5 integrin (A) or FGFR2 antibodies (B), resolved by SDS-PAGE and blotted with anti-FGFR2 or anti-5 integrin antibodies. (C) Control and FGFR2 mutant osteoblasts cultured on glass coverslips overnight in DMEM containing 10% FCS were fixed and stained with an anti-5 integrin subunit antibody (green) or anti FGFR2 polyclonal antibody (red). Note the colocalization (yellow) of the 5 integrin subunit and FGFR2 in membrane ruffle regions (arrows). Bar, 10 µm.