Fig. 5. Colocalization of 5 integrin subunit and Cbl in osteoblasts. Control and FGFR2 mutant osteoblasts were cultured overnight on glass coverslips overnight in DMEM containing 10% FCS. After fixation, the cells were stained with an anti-5 integrin subunit (green) or anti-Cbl (red). Note the colocalization of the 5 integrin subunit with Cbl in membrane ruffle regions (yellow). Bar, 10 µm.