Fig. 7. Transfection with 5 integrin plasmid rescues osteoblast attachment on fibronectin in FGFR2 mutant osteoblasts. (A) FGFR2 mutant osteoblasts were transfected with 5 plasmid (pcDNA-5) or the empty vector (pcDNA). After 24 hours, cell lysates were immunoprecipitated (IP) with anti-5 integrin, resolved by SDS-PAGE and blotted with 5 integrin antibody. (B) FGFR2 mutant osteoblasts transfected with 5 integrin plasmid or empty vector were plated on fibronectin-coated dishes, cultured for 48 hours and the number of adherent cells was counted. Data are the mean±s.e.m. of six wells; a and b indicate a significant difference with control and pcDNA-mutant cells, respectively (P<0.05).