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Fig. 4. Effect of PAF on erythrocyte cell volume. (A) Histograms of forward-scatter in FACS analysis of a representative experiment of erythrocytes incubated for 24 hours in Ringer solution containing 0.3% ethanol as a control (control), in Ringer solution + PAF16 (1 µM PAF16; 3.8 µM PAF16) and in Ringer solution + PAF18 (1 µM PAF18; 3.6 µM PAF18). Numbers depict the geometric mean of the forward scatter of the cell population. (B) Erythrocyte forward scatter in FACS analysis after a 24-hour treatment with Ringer solution containing 0.3% ethanol (control) and after incubation with 3.8 µM PAF16 or 3.6 µM of PAF18 (means ± s.e.m., n=8). * indicates significant difference from control (ANOVA, using Dunnett's test as post-hoc test; P≤0.05). (C) Forward scatter in FACS analysis of erythrocytes (means ± s.e.m., n=6-8), treated for 24 hours with different concentrations (0.45-3.8 µM) of PAF16 ({blacktriangleup}) or PAF18 ({square}). Controls (0 µM PAF) contained appropriate amounts of ethanol. Additionally, erythrocytes were preincubated with 25 µM quinacrine for 3 hours and PAF16 ({blacktriangleup}) was diluted to the appropriate concentrations (125-2000 nM) from 2 mM DMSO stock solutions. Forward scatter in FACS analysis (means ± s.e.m., n=3-5) is depicted in the insert and is given in arbitrary units. * indicates significant difference from controls (ANOVA, using Dunnett's test as post-hoc test; P≤0.05).