Fig. 5. Influence of Triton X-100 on cell number and erythrocyte-annexin-binding. (A) Number of erythrocytes seeded at 0.2% haematocrit and treated for 24 hours with different concentrations (0.0003%-0.03%) of Triton X-100 (). The number of erythrocytes after treatment is given in 10⁶ cells/ml (arithmetic means ± s.e.m., n=6). Controls (0% Triton X-100) were treated with Ringer solution. * indicates significant difference from controls (ANOVA, using Dunnett's test as post-hoc test; P0.05). (B) Number of FITC-Annexin-V-binding cells per 20,000 cells as obtained by FACS analysis of erythrocytes after a 24-hour treatment with Ringer solution or after incubation with 0.0003% or 0.001% Triton X-100 (arithmetic means ± s.e.m., n=6). As a positive control, erythrocytes were treated with 1 µM PAF16 diluted from a 2 mM DMSO stock solution. * indicates significant difference from vehicle-treated control (ANOVA using Dunnett's test as post hoc test; P0.05).