Fig. 5. Localization of Necl-1 at non-junctional cell-cell contact sites in peripheral myelinated nerve fibers. Samples were stained with anti-Necl-1 pAb followed by immunogold electron microscopy with the silver-enhanced immunogold method. (A) Axons with myelin sheath. (B) Nodes of Ranvier. (B') Schematic drawing of B. M, myelin sheath; Ax, axon of peripheral nerve fiber; S, cellular process of Schwann cells. Yellow arrows indicate contact sites of cellular processes of Schwann cells. Bars, 250 nm. The results shown are representative of three independent experiments.