Fig. 1. Representative western-blot analysis of caspase-3 cleavage in a time-course experiment of p25 expression. NGF-differentiated (7 days) mock and p25 cells were treated (+) or not (–) with tetracycline for 24 hours or 48 hours, and lysates were analysed using anti-caspase-3 antibody that recognizes the full-length protein (35 kDa, arrow) and the large fragments resulting from its cleavage (19/17 kDa, arrowheads). As positive control, lysates derived from cells treated (+) or not (–) with 1 µM staurosporine (SSP) were used.