Fig. 7. Location of GFP-SRP19 in partially dissociated nucleoli. At 5 hours after injecting GFP-SRP19 into the cytoplasm of oocytes, GV spreads were prepared in the absence of Mg²⁺ and either examined unfixed (A-C) or after storing the preparations for 18 hours in 70% ethanol (D-F). Distended nucleolar substructure is evident from the phase-contrast images (A,D), with globular dense fibrillar component organized around individual DAPI-staining fibrillar centres (B,E). The GFP signal remained located at the dense fibrillar component (C,F) and was absent from chromosomes (D-F) and other nuclear structures (not shown).