Fig. 8. Immunostaining of amplified nucleoli with antibodies to SRP19. GV spreads were prepared in low-salt medium without Mg²⁺. Nucleoli (No), Cajal bodies (CB) and B-snurposomes were identified by phase-contrast (A) and DAPI staining (B), but only the nucleoli were immunostained using chicken anti-SRP19 with FITC-conjugated anti-chicken IgG as a secondary antibody (C). The centres of the stained structures are seen to be relatively free of fluorescence. (D-F) The DAPI staining (D) and the immunostaining (E) are merged (F), with the DAPI computationally coloured red and yellow indicating spatial coincidence of the two signals.