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Fig. 3. (A,B) Western-blot analysis of membrane proteins isolated from wt CHO, CHO(LpR), CHO(LpR1-790LDLR791-839) and ldlA(LpR1-342LDLR293-839) cells (lanes 1-4, respectively). The samples were subjected to SDS-PAGE under reducing (A) or non-reducing (B) conditions and blotted onto PVDF membrane. The wt LpR, LpR1-790LDLR791-839 and LpR1-342LDLR293-839 were detected with anti-LpR antibody 2189/90. (C) Membrane proteins of LDLR-deficient ldlA (lane 1), wt CHO (lanes 2 and 8), ldlA(LDLR) (lane 3), ldlA(LDLRH562Y) (lane 4), ldlA(LDLRH562N) (lane 5), ldlA(LDLR1-292LpR343-850) (lanes 6, 7) and CHO(LpR) cells (lane 9) were subjected to SDS-PAGE under non-reducing conditions and blotted onto PVDF membrane Lanes 1-6 were incubated with anti-LDLR antibody 121 and lanes 7-9 with anti-LpR antibody 9218. The size markers (in kDa) are indicated on the left (A-C) and right (C) of the blots.