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Fig. 1. Large TIN2 domains are present in HMECs organized into acini. (A) Immunostaining for TIN2 (green) and PNA FISH for telomeres (red) in S1 acini in 3D culture. (B) Immunostaining for TIN2 (green) in a biopsy from normal breast tissue. (C) Dual immunostaining for TIN2 (red) and TRF2 (green) in the nuclei of proliferating 184 HMECs. Colocalization of TIN2 and TRF2 appears yellow. (D) Dual immunostaining for TIN2 (red) and V5 (green) in the nuclei of acini formed by 184 HMECs expressing V5-tagged TIN2 in 3D culture. Nuclei are counterstained with DAPI (blue) in A, C and D, and propidium iodide (red) in B. Images in B and D are confocal sections of acini containing several nuclei. Arrowheads indicate large TIN2 domains. Bar, 5 µm.