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Fig. 3. Formation of large TIN2 domains in growth-arrested cells is independent of the differentiation status. (A) Large TIN2 domains (arrowheads) revealed by immunostaining (red) in correctly polarized S1 cells cultured in 3D laminin-rich ECM (S1-Matrigel), S1 cells cultured in 3D collagen I (S1-Collagen I) that display altered polarity, and growth-arrested (EGF-deprived) S1 cells cultured as a monolayer on plastic. Nuclei are counterstained with DAPI (blue). (B) Immunostaining for TIN2 (yellow) in growth-arrested 184 cells cultured as a monolayer on plastic. The superimposed phase-contrast image shows the nucleoli as dark gray subnuclear structures. Arrows indicate large TIN2 domains located next to nucleoli. (C) Dual immunostaining for HP 1{gamma} (green) and TIN2 (red) in growth-arrested 184 cells cultured as a monolayer on plastic. Arrowheads indicate overlapping (yellow) HP 1 staining and large TIN2 domains. Nuclei were counterstained with DAPI (blue). Bar, 5 µm.