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Fig. 5. Colocalization of HBZ mutants deleted of their N- and C-terminal domains with C23 in the nucleoli. COS cells transfected with the mutants HBZ-BR1/DBD, HBZ-BR2/DBD and HBZ-BR2/BR1 fused to EGFP (column EGFP) were labeled with a mouse anti-C23 antibody (column C23) and detected using goat anti-mouse IgG antibody coupled to TRITC. Analysis of the green, red and merged fluorescence was performed with a fluorescence microscope. Magnification, 1200 x.