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Fig. 6. In vivo expression of the Myc-tagged HBZ mutants. COS cells were transfected with pcDNA3.1(–)/Myc-His expressing HBZ and the mutants HBZ-{Delta}ZIP, HBZ-BR2/BR1/DBD and HBZ-{Delta}AD fused to the Myc epitope. After transfection in COS cells, expression of the different mutants was checked by western blotting (top) and their localization analyzed by fluorescence microscopy using the mouse anti-Myc antibody (bottom). For immunofluorescence analysis, the anti-Myc antibody was detected with goat anti-mouse IgG antibody coupled to FITC. Magnification, 1500 x.