Fig. 7. Analysis of HBZ subnuclear localization. Localization of the transiently expressed HBZ-EGFP was analyzed by immunofluorescence microscopy. (A) COS cells cotransfected with HBZ-EGFP and PML protein expression vector were labeled with a mouse anti-PML protein antibody and detected using goat anti-mouse IgG antibody coupled to TRITC. Analysis of the green, red and merged fluorescence was performed with a fluorescence microscope. (B) The same approach was performed with HBZ-EGFP-transfected COS cells to localize endogenous SC35 labeled with a mouse anti-SC35 antibody, and (C) with COS cells cotransfected with pEGFP-HBZ and pSG-Tax. Tax wax labeled with a culture supernatant of the anti-Tax 168A51-42 hybridoma. Magnification, 1500 x.