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Fig. 3. Visualization of the cellular localization of Syt I and Syt II chimeric proteins. (A) RBL cells transiently double transfected with HA-Syt I/Syt II and T7-Syt I cDNA were grown on glass coverslips and labeled with polyclonal anti-HA and monoclonal anti-T7 antibodies followed by Rhodamine-conjugated donkey anti-mouse and FITC-conjugated donkey anti-rabbit IgG. (B) RBL cells transiently transfected with HA-Syt I/Syt II cDNA were grown on glass coverslips and double stained with polyclonal anti-HA and monoclonal anti-serotonin antibodies followed by Rhodamine-conjugated donkey anti-rabbit and FITC-conjugated donkey anti-mouse IgG. (C) RBL cells, transiently transfected with HA-Syt II (1-183aa)/Syt I (a), HA-Syt II (1-86aa)/Syt I (b) or HA-Syt II (1-60aa)/Syt I (c) cDNA, were grown on glass coverslips and stained with monoclonal anti-HA antibodies. Cells were processed for immunofluorescent staining and visualized by confocal microscopy. Bar, 10 µm.