Fig. 6. Effect of inhibitors of internalization on HA-Syt I/Syt II trafficking. RBL cells transiently transfected with HA-Syt I/Syt II cDNA were grown on glass coverslips for 1 hour and subsequently incubated for 2 hours at 20°C (a). Cells were then warmed to 37°C for 3 hours in the absence (b) or presence of chloroquine (100 µM) (c) or sucrose (0.45 M) (d). Cells were subsequently stained with monoclonal anti-HA antibodies followed by Cy3-conjugated secondary antibody. Cells were processed for immunofluorescence and visualized by laser confocal microscopy. Bar, 5 µm.