Fig. 7. Cellular localization of GFP-tagged Syt II. RBL cells stably transfected with Syt II-GFP (A) or Flag-Syt II-GFP (A,B) were grown on glass coverslips and either left untreated (A) or were serum starved for 1 hour and subsequently incubated for the indicated time periods with Texas Red-conjugated Tfn (50 µg/ml) (B). Cells were fixed and visualized by laser confocal microscopy. Bar, 10 µm.