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Fig. 3. Presence of actin isoforms in the RLSs. (A) Overlay images of EGFP fluorescence (green) and (a) staining of ß-cytoplasmic actin (red) or (b) {gamma}-cytoplasmic actin (red) in non-treated {alpha}-SMA-EGFP-transfected cells are shown. Scale bar, 10 µm. (B) Cells were treated for 30 minutes with SMA-FP, fixed with 1% paraformaldehyde for 10 minutes followed by methanol incubation for 3 minutes and stained with either (b) anti ß-cytoplasmic actin antibody, (e) {gamma}-cytoplasmic actin antibody or (h) Alexa 568-phalloidin. (k) The distribution of rhodamine-labeled SMA-FP in {alpha}-SMA-EGFP-transfected cells was also investigated. {alpha}-SMA-EGFP patterns are shown in images a, d, g, and j and colocalization was visualized by confocal microscopy. Overlay images are shown in c, f, i and l. Bar, 10 µm.