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Fig. 1. Apoptosis of primary cells. {gamma}SG-deficient myotubes on rigid substrate exhibited greater apoptosis than wild-type C57 myotubes at day 7. Both C57 and {gamma}SG–/– cells were split, plated in parallel on soft collagen-substrates or rigid IPN-patterned coverslips, and later tested for apoptosis by TUNEL assay at day 2 and day 7. The mean intensity of nuclei for the cells on coverslips was divided by the mean intensity for the cells grown on the collagen-substrates for each day. Error bars represent the s.e.m. of the intensities of at least 50 cells. The relative intensity for {gamma}SG–/– at day 7 was significantly greater (17-fold) (P<0.001) than the relative intensity at day 2, indicating that {gamma}SG–/– cells cultured on rigid substrate apoptosed to a far greater extent than normal cells under normal conditions.