Fig. 2. Localization of GFP-vinculin constructs in B16-F1 cells. B16-F1 populations were seeded on laminin and allowed to attach for 6 hours. Thereafter, cells were fixed and stained for paxillin and the actin cytoskeleton. Localization of GFP-vinculin constructs, paxillin and F-actin in exemplary cells are shown individually (greyscale) as well as on merged images of GFP-vinculin (green), paxillin (blue), F-actin (red) and enlargements of the regions indicated. Scale bars, 10 µm (overviews); 2 µm (insets).