Fig. 5. Locomotory activity and velocity of B16-F1 populations in collagen gels. Migration of B16-F1 cells expressing vinculin variants was analysed by time-lapse video microscopy. The number and velocity of motile cells were calculated from tracking data (30 cells per vinculin variant, n=3 repeats). (A) Representative tracks of cells expressing vinculin-wt or vinculin-LD, respectively. Columns represent (B) average of motile cells (mean±s.d.) and (C) average velocity of active cells (mean±s.d.) of the population between 6-15 hours (constant phase).