Fig. 2. Sas2p and Sas5p interacted with the importins Pse1p and Kap123p. (A) Domains in Pse1p. The blue bar indicates the importin ß homology domain containing an Armadillo repeat (ARM, red). The C-terminal fragments of Pse1p interacting with Sas5p in the two-hybrid assay are illustrated by black lines. (B) Coimmunoprecipitation analysis of Sas5p-myc₉/Pse1p-HA₃, Sas5p-myc₉/Kap123p-HA₃, myc₆-Sas2p/Pse1p-HA₃ and myc₆-Sas2p/Kap123p-HA₃ interactions. Bound proteins were separated on 12.5% SDS polyacrylamide gels, immunoblotted and probed with an -HA antibody. Pse1p-HA₃ coimmunoprecipitated with Sas5p-myc₉ or myc₆-Sas2p (upper panels), and Kap123p-HA₃ co-precipitated with Sas5p-myc₉ or myc₆-Sas2p (lower panels).