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Fig. 1. Schematic representation of the hypoglycosylated CPY* variants used in this study. (A) The four glycosylation sites of CPY* are numbered according to their positions in the mature enzyme. G255R is the site of the CPY* mutation. (B) Hypoglycosylated CPY* variants are assigned a four-digit binary code, with 1 specifying the presence and 0 the absence of a glycan at a particular position (Asn13, Asn87, Asn168 or Asn368, respectively).