Fig. 5. CTG* degradation depends on N-glycans and Htm1p. CTG* and CTG*0000 were expressed from a low-copy-number vector in isogenic wild-type and
htm1 strains. Proteins were labeled with [35S]-methionine, chased at the indicated time points and immunoprecipitated using anti-CPY antibodies. Data are derived from four (CTG*,
htm1CTG*0000) or five (CTG*0000,
htm1CTG*) independent experiments.