Fig. 6. Spheroids of live AS-cav-1 HCT 116 cells degrade less DQ-collagen IV both intracellularly (long arrow) and pericellularly (arrowhead) than do spheroids of live control HCT 116 cells. (A) Cells were plated on DQ-collagen-IV/Matrigel-coated coverslips and imaged at 48 hours. Fluorescent images of DQ-collagen-IV degradation products (green), taken using an extended depth of focus, are superimposed on phase images of live spheroids of control and AS-cav-1 HCT 116 cells. Bar, 10 µm. Images are representative of at least three experiments. (B) Fluorescent intensity (intracellular plus pericellular) was measured using Metamorph 6.0 software and is expressed as average intensity. The graph is representative of at least three experiments and is presented as mean ± s.d. **P<0.01.