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Fig. 7. Mean FRET efficiency as a function of relative concentration ratio of donor/acceptor (Cx-ECFP/Cx-EYFP) and acceptor fluorescence intensity level for different combinations of connexins. The decrease of FRET efficiency with the increase of donor/acceptor concentration ratio, but its insensitivity to the increase of absolute acceptor level, indicate that donors and acceptors are clustered into connexons.